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German Alexander Zverev’s 10-match winning streak ended with defeat by unseeded American Francis Tiafoe in the second round of the Cincinnati Open.
Zverev, 20, has moved up to seventh in the world rankings following victory over Roger Federer in Montreal.
But he lost 6-4 3-6 4-6 to the 19-year-old Tiafoe, the world number 87.
In the women’s event, world number nine Venus Williams was also knocked out in the second round by Australian qualifier Ashleigh Barty.
Barty, ranked 48 in the world, beat the Wimbledon finalist 6-3 2-6 6-2 to earn her first victory over a top-10 player.
Williams, 37, served up six double faults in an error-strewn performance.
Barty, 21, will next face world number five Caroline Wozniacki in the third round.
“It’s an honour to walk out on the the same court as Venus, to play against her and share the court with her,” Barty said.
“She’s a genuine champion of the game and has, I think, paved the way for us young players coming through so to play like I did, I’m really excited.”
Dane Wozniacki, one of four women who could replace Karolina Pliskova as world number one if results go her way this week, beat Russia’s Elena Vesnina 6-2 6-4 to progress.
But German Angelique Kerber, who could have moved up from third to first in the rankings, was beaten in a third-set tie-break by Russian Ekaterina Makarova, who converted her eighth match point to win 6-4 1-6 7-6 (13-11).
Simona Halep and Elina Svitolina, the final two women in contention for the number one spot, are yet to play their second-round matches.
Elsewhere, American Madison Keys saw off Russia’s Daria Kasatkina in 6-2 6-1, where she will face Wimbledon champion and fourth seed Garbine Muguruza of Spain.
Del Potro form continues
In the men’s event, Bulgarian Grigor Dimitrov recorded his 30th match win of the year to knock out Spain’s Feliciano Lopez 7-6 (7-5) 6-4 and will next face Juan Martin del Potro of Argentina.
Del Potro, who on Tuesday upset 10th seed Czech Tomas Berdych, defeated American qualifier Mitchell Krueger 6-4 6-4 in a hard-fought encounter.
The world number 30 saved five break points at 3-3 in second set and clinched victory on his fourth match point.
Australian Nick Kyrgios saw off Ukraine’s Alexandr Dolgopolov 6-3 7-6 (8-6) to advance to the third round, where he will face unseeded Croatian Ivo Karlovic.
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